Expression of APikL effector family members in the blast fungus varies during plant infection
| Main Authors: | Langner, Thorsten, Win, Joe, Kamoun, Sophien |
|---|---|
| Format: | Document publication-technicalnote Journal |
| Bahasa: | eng |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5531601 |
Daftar Isi:
- This Technical Note complements our paper on the molecular evolution of the heavy-metal associated (HMA) domain binding effector candidate APikL2 and other APikL family members in the blast fungus Magnaporthe oryzae [1]. We identified a six gene family of lineage-specific effector candidates with sequence similarity to the well-characterized effector AVR-Pik. APikL2 and other members of the AVR-Pik like (APikL) family display potentially adaptive mutations in two distinct HMA-binding interfaces that influence binding specificity, similarly to mutations observed in AVR-Pik. However, whereas the function of AVR-Pik during infection is well documented, the role of other APikL family members remains to be determined, mainly because no resistance genes are known that recognize these effectors. Here, we investigated publicly available RNA-sequencing (RNA-seq) datasets to gain insights into the expression profiles of the APikL family members AVR-Pik, APikL2 and APikL5 in the rice infecting isolate ina168 and field transcriptomics samples of the 2016 wheat blast outbreak in Bangladesh. This analysis showed that expression of APikL family members varies in the examined datasets. While AVR-Pik is expressed in the rice infecting isolate ina168 during early infection, no reads mapped to APikL2. Similarly, no read coverage was observed at the APikL2 or APikL5 loci in field samples of wheat blast from Bangladesh. From this, we conclude that expression profiles of APikL members vary. However, to this date we cannot rule out if the other members of the APikL family are expressed in certain conditions, tissues, or host-species.