Relationship between lipid peroxidation and antioxidants in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes
| Main Author: | Verveha, Bоhdana |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2021
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| Online Access: |
https://zenodo.org/record/5213931 |
Daftar Isi:
- Verveha Bоhdana. Relationship between lipid peroxidation and antioxidants in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes. Journal of Education, Health and Sport. 2021;11(06): 249-258. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.06.027 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.06.027 https://zenodo.org/record/5213931 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 21.05.2021. Revised: 03.06.2021. Accepted: 30.06.2021. Relationship between Lipid peroxidation and antioxidants in RATS WITH EXPERIMENTAL ACUTE generalized PERITONITIS AGAINST THE BACKGROUND OF STREPTOZOTOCIN-INDUCED DIABETES Bоhdana Verveha Danylo Halytsky Lviv National Medical University, Ukraine danaverveha@gmail.com; https://orcid.org/0000-0002-7463-5899 There are evidence that oxidative stress is involved in the pathogenesis of the acute generalized peritonitis and diabetes mellitus. The aim of study was to examine levels of the products of lipid peroxidation and the antioxidants in rats with experimental acute generalized peritonitis against the background of streptozotocin-induced diabetes. Material and methods. The experiment was performed on 66 adult white male rats weighing 220-300 g. Diabetes mellitus in experimental animals was simulated by a single intraperitoneal injection of streptozotocin (Sigma) at the dose of 60 mg/kg. Acute generalized peritonitis was induced with 10% faecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method. The thiobarbituric acid reactive substances (TBARS), lipid hydroperoxides (LOOHs), superoxide dismutase (SOD) activity, catalase (CAT) and ceruloplasmin (CP) were estimated and compared. Our results showed that during all stages of development of acute generalized peritonitis on the background of streptozotocin-induced diabetes there is the LOOHs decrease and the TBARS increase. We observed statistically significant inverse correlations between the level of LOOHs and SOD in the animals of subgroup 1 (r = -0.88; p <0.05) and inverse correlations between the TBARS level and the SOD level in the subgroup 3 of animals (r = -0.74; p <0.05). We also found a statistically significant inverse correlation between the CAT level and the TBARS level (r = -0.86; p <0.05) and between the CP level and the TBARS level in the blood of animals in the subgroup 3 (r = -0.87; p <0.05). Negative statistically significant correlations between the TBARS level and the antioxidants (SOD, CAT, CP) in the blood of animals with acute generalized peritonitis on the background of streptozotocin-induced diabetes indicate a predictor role of lipid peroxidation processes in the depletion of antioxidant resources. Key words: acute generalized peritonitis; streptozotocin-induced diabetes; lipid peroxidation; antioxidant defense system; correlation analysis.