BISPHENOL A EXPOSURE IN ZEBRAFISH (DANIO RERIO) AS A POTENTIAL ENVIRONMENTAL TOXIN MODEL OF AUTISM SPECTRUM DISORDER
| Main Author: | Scaramella, Courtney |
|---|---|
| Format: | info publication-thesis Journal |
| Bahasa: | eng |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5111097 |
Daftar Isi:
- As the diagnostic rate of autism spectrum disorder continues to rise, the likelihood of an underlying environmental influence increases. Due to its known endocrine and epigenetic effects, as well as its ubiquity, we believe bisphenol A (BPA) to be an ideal candidate for further use and investigation as an environmental toxin model of autism. With its reduced neural complexity, genetic tractability, and ability to be exposed to pharmacological agents in bath with precise control, we chose to use larval zebrafish in our experiments to find the ideal concentration of BPA that would potentially elicit autistic phenotypes without causing any major additional health problems. Chronic BPA exposure over the first five days of development showed that concentrations of 30 mM or higher led to high rates of severe physical deformities in 6 dpf zebrafish. Additionally, while the highest BPA concentration tested (50 mM) significantly increased mortality, five-day exposure to lower doses (10 & 20 mM) had no significant effects on fish health or survivability.