Influence of disposable relaparotomy and programmed sanation on the expression and dynamics of clusters determinations on immunocompetent cells of patients with abdominal sepsis caused by severe peritonitis
| Main Authors: | Polyovyy, V., Dzyubanovsky, I., Kovalchuk, A., Dzyubanovsky, O., Dzhyvak, V. |
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| Format: | Article Journal |
| Terbitan: |
, 2021
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| Online Access: |
https://zenodo.org/record/5035199 |
Daftar Isi:
- Polyovyy V., Dzyubanovsky I., Kovalchuk A., Dzyubanovsky O., Dzhyvak V. Influence of disposable relaparotomy and programmed sanation on the expression and dynamics of clusters determinations on immunocompetent cells of patients with abdominal sepsis caused by severe peritonitis. Journal of Education, Health and Sport. 2021;11(04): 157-166. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2021.11.04.016 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2021.11.04.016 https://zenodo.org/record/5035199 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2021; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 26.02.2021. Revised: 31.03.2021. Accepted: 30.04.2021. INFLUENCE OF DISPOSABLE RELAPAROTOMY AND PROGRAMMED SANATION ON THE EXPRESSION AND DYNAMICS OF CLUSTERS DETERMINATIONS ON IMMUNOCOMPETENT CELLS OF PATIENTS WITH ABDOMINAL SEPSIS CAUSED BY SEVERE PERITONITIS V. P. Polyovyy1, I. Ya. Dzyubanovsky2, A. A. Kovalchuk2, O. I. Dzyubanovsky2, V. H. Dzhyvak2 1Bukovinian State Medical University 2I. Horbachevsky Ternopil National Medical University Polyovyy V. P. http://orcid.org/0000-0002-4345-9802 Dzyubanovsky I. Ya. http://orcid.org/0000-0001-8852-3938 Kovalchuk A. A. https://orcid.org/0000-0003-1394-7546 Dzyubanovsky O. I. https://orcid.org/0000-0003-4343-2797 Dzhyvak V. H. https://orcid.org/0000-0002-4885-75861 Abstract Relaparotomy in the treatment of postoperative complications of abdominal surgery remains a difficult problem of modern surgery. To evaluate the prognostic value of expression clusters of determination on immunocompetent cells of patients, the dynamics of HLA-DR + molecules and cytokines in the blood of patients with abdominal sepsis caused by severe peritonitis, as well as abscesses and phlegmons for postoperative period. Materials and methods. The study was conducted in 40 patients diagnosed with abdominal sepsis in the period from 2017-2020, which was treated in medical institutions of Chernivtsi and Ternopil in Ukraine, and which were divided into control and research groups. The control group consisted of 17 patients without signs of abdominal sepsis and acute surgical pathology. Determination of the expression of determination clusters such as CD11a, CD162, CD95, CD16 on immunocompetent cells, and the study of the dynamics of expression of HLA-DR + molecules and the content of cytokines IL-2, IL-4, IL-6 in the blood of patients was performed in the postoperative period. Both groups were representative by age, sex, comorbidities, risk factors. Determination of experimental parameters in the blood of patients was performed in the postoperative period (the results were evaluated before surgery, on the first, third, seventh, and on the fourteenth day of the postoperative period). Research results and their discussion In patients with abdominal sepsis caused by severe peritonitis, the expression of HLA-DR + molecules on immunocompetent cells increases, which to some extent indicates an intensification of γ-interferon synthesis. At the same time, there is a sharp decrease in the content of IL-2 in the blood - the main regulator of a specific immune response. Keywords. Peritonitis; relaparotomy; clusters; cytokines; sepsis.