Data from: Super-resolution imaging of a 2.5 kb non-repetitive DNA in situ in the nuclear genome using molecular beacon probes
| Main Authors: | Ni, Yanxiang, Cao, Bo, Ma, Tszshan, Niu, Gang, Huo, Yinghuo, Huang, Jiandong, Chen, Danni, Liu, Yi, Yu, Bin, Zhang, Michael Qiwei, Niu, Hanben, Huo, Yingdong |
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| Format: | info dataset Journal |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5007387 |
Daftar Isi:
- High-resolution visualization of short non-repetitive DNA in situ in the nuclear genome is essential for studying looping interactions and chromatin organization in single cells. Recent advances in fluorescence in situ hybridization (FISH) using Oligopaints probes enabled super-resolution imaging of genomic domains with a resolution limit of 4.9 kb. To target shorter elements, we developed a simple FISH method that uses only molecular beacon (MB) probes to facilitate the probe-target binding, while minimizing non-specific fluorescence. We used three-dimensional stochastic optical reconstruction microscopy (3D-STORM) and optimized the imaging conditions to efficiently distinguish sparsely distributed Alexa-647 from background cellular autofluorescence. Utilizing 3D-STORM and 29-34 individual MB probes, we observed 3D fine-scale nanostructures of 2.5 kb integrated or endogenous unique DNA in situ in the human or mouse genome, respectively, demonstrating the capability of MB-based FISH in visualizing a so far shortest and non-repetitive genomic sequence in 3D at super-resolution.
- Source data for Figure 4a-cell IIIRevised2_figure4a_right_raw_data.rarSource data for Figure 4a-cell IIRevised2_figure4a_middle_raw_data.rarSource data for Figure 4a-cell IRevised2_figure4a_left_raw_data.rarSource data for Figure 5d-cell IRevised2_figure5d_cell1_raw_data.rarSource data for Figure 5d-cell IIRevised2_figure5d_cell2_raw_data.rarSource data for Figure 5d-cell IIIRevised2_figure5d_cell3_raw_data.rarSource data for Figure 5d-cell IVRevised2_figure5d_cell4_raw_data.rarSource data for Figure 5d-cell VRevised2_figure5d_cell5_raw_data.rarSource data for Figure 5d-cell VIRevised2_figure5d_cell6_raw_data.rarREADME