Data from: Significant differences in maternal carotenoid provisioning and effects on offspring fitness in Chinook salmon colour morphs
| Main Authors: | Lehnert, Sarah J., Garver, Kyle A., Richard, Jon, Devlin, Robert H., Lajoie, Celine, Pitcher, Trevor E., Heath, Daniel D. |
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| Format: | info dataset Journal |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5001480 |
Daftar Isi:
- In oviparous species, maternal carotenoid provisioning can deliver diverse fitness benefits to offspring via increased survival, growth, and immune function. Despite demonstrated advantages of carotenoids, large intra‐ and interspecific variation in carotenoid utilization exists, suggesting trade‐offs associated with carotenoids. In Chinook salmon (Oncorhynchus tshawytscha), extreme variation in carotenoid utilization delineates two colour morphs (red and white) that differ genetically in their ability to deposit carotenoids into tissues. Here, we take advantage of this natural variation to examine how large differences in maternal carotenoid provisioning influence offspring fitness. Using a full‐factorial breeding design crossing morphs and common‐garden rearing, we measured differences in a suite of fitness‐related traits, including survival, growth, viral susceptibility, and host response, in offspring of red (carotenoid‐rich eggs) and white (carotenoid‐poor eggs) females. Eggs of red females had significantly higher carotenoid content than those of white females (6X more); however, this did not translate into measurable differences in offspring fitness. Given that white Chinook salmon may have evolved to counteract their maternal carotenoid deficiency, we also examined the relationship between egg carotenoid content and offspring fitness within each morph separately. Egg carotenoids only had a positive effect within the red morph on survival to eyed‐egg (earliest measured trait), but not within the white morph. While previous work shows that white females benefit from reduced egg predation, our study also supports a hypothesis that white Chinook salmon have evolved additional mechanisms to improve egg survival despite low carotenoids, providing novel insight into evolutionary mechanisms that maintain this stable polymorphism.
- Egg carotenoid contentEgg carotenoid content in ug/g for unfertilized eggs from Chinook salmon femalesCarotenoid_content.csvRelative gene expression dataRelative gene expression data for all fish in control and treatment tanksAll_relative_expression_data.csvSurvival to eyed-egg stageData for survival from fertilization to eyed-egg stage. All eggs were counted (dead and alive) to calculate survival for each cell (replicates for each cross). Data were converted to binary data for analyses. A total of 56 families were used in this analysis.egg survival for binary-56 families.csvSurvival from eyed-egg to frySurvival from eyed-egg to fry measured by alive and dead offspring. Survival values are provided for each family (n=56). Data were converted to binary format for analyses.egg to fry data survival-CAER.csvEarly fry survivalData for early fry survival (from ponding to February). Survival data are provided for each family. Data were transformed to binary format for analyses.early fry survival -CAER.csvLate fry survivalData for late fry survival (February to June). Data are provided for each tank and were transformed to binary format for analyses.late fry survival-CAER.csvWeight and length data for fryWeight (wt) and fork length (FL) data for fry in December, February, April, and July. A total of 15 fish were randomly sampled at each date (not same fish each time). Missing data points within a family indicate that no additional fish were alive in the family at that time point (or after that point).Ponding_data_Ques2015_caer.csvIHNV survival challengeSurvival data (dead and alive fish) for IHNV and mock challenge. Survival was measured over 35 days after exposure to the live IHNV virus or exposure to mock conditions.