Data from: Genetic tradeoffs and conditional neutrality contribute to local adaptation
| Main Authors: | Anderson, Jill T., Lee, Cheng-Ruei, Rushworth, Catherine A., Colautti, Robert I., Mitchell-Olds, Thomas |
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| Format: | info dataset Journal |
| Terbitan: |
, 2011
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4971019 |
Daftar Isi:
- Divergent natural selection promotes local adaptation and can lead to reproductive isolation of populations in contrasting environments; however, the genetic basis of local adaptation remains largely unresolved in natural populations. Local adaptation might result from antagonistic pleiotropy, where alternate alleles are favored in distinct habitats, and polymorphism is maintained by selection. Alternatively, under conditional neutrality some alleles may be favored in one environment but neutral at other locations. Antagonistic pleiotropy maintains genetic variation across the landscape; however, there is a systematic bias against discovery of antagonistic pleiotropy since the fitness benefits of local alleles need to be significant in at least two environments. Here, we develop a generally applicable method to investigate polygenic local adaptation and identify loci that are the targets of selection. This approach evaluates allele frequency changes after selection at loci across the genome to distinguish antagonistic pleiotropy from conditional neutrality and deleterious variation. We investigate local adaptation at the QTL-level in field experiments, in which we expose 177 F6 recombinant inbred lines (RILs) and parental lines of Boechera stricta (Brassicaceae) to their parental environments over two seasons. We demonstrate polygenic selection for native alleles in both environments, with 2.8% of the genome exhibiting antagonistic pleiotropy, and 8% displaying conditional neutrality. Our study strongly supports antagonistic pleiotropy at one large-effect flowering phenology QTL (nFT): native homozygotes had significantly greater probabilities of flowering than foreign homozygotes in both parental environments. Such large-scale field studies are essential to elucidate the genetic basis of adaptation in natural populations.
- Flowering status data for DryadThese are individual-level data on the flowering success of experimental individuals (Recombinant Inbred Lines and parental lines) outplanted into Colorado and Montana field gardens in September 2008 and 2009. In this file, site indicates the common garden where an individual was planted. Cohort refers to the year in which we assessed fitness (growing season of 2009 or 2010). Family identifies the Recombinant Inbred Line or Parental Line family. Block indicates the block into which an individual was planted in each garden. Flowered indicates whether an individual flowered (1) or not (0). We've included two columns for the genotype at the nFT QTL. The first includes heterozygotes, and the second excludes them. Finally %CO is the proportion of the genome with Colorado alleles, excluding the nFT region. Please see the manuscript for additional information.