Data from: Somatic deleterious mutation rate in a woody plant: estimation from phenotypic data
| Main Authors: | Schoen, Daniel J., Bobiwash, Kyle, Schultz, Stewart T. |
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| Format: | info dataset Journal |
| Terbitan: |
, 2013
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4945351 |
Daftar Isi:
- We conducted controlled crosses in populations of the long-lived clonal shrub, Vaccinium angustifolium (lowbush blueberry) to estimate inbreeding depression and mutation parameters associated with somatic deleterious mutation. Inbreeding depression level was high, with many plants failing to set fruit after self-pollination. We also compared fruit set from autogamous pollinations (pollen collected from within the same inflorescence) to fruit set from geitonogamous pollinations (pollen collected from the same plant but from inflorescences separated by several meters of branch growth). The difference between geitonogamous versus autogamous fitness within single plants is referred to as "autogamy depression" (AD). AD can be caused by somatic deleterious mutation. AD was significantly different from zero for fruit set. We developed a maximum likelihood procedure to estimate somatic mutation parameters from AD, and applied it to geitonogamous and autogamous fruit set data from this experiment. We infer that on average, approximately three sublethal, partially dominant somatic mutations exist within the crowns of the plants studied. We conclude that somatic mutation in this woody plant results in an overall genomic deleterious mutation rate that exceeds that measured to date for annual plants. Some implications of this result for evolutionary biology and agriculture are discussed.
- Data for estimation of inbreeding depressionData from 24 fields (12 putative clones per field). Each clone was self- and cross-pollinated by hand. These treatments were conducted at the inflorescence level. At the time of pollination, the number of flowers per inflorescence was counted. Later, we returned to these clones and counted the number of fruits (berries) per clone. The data are proportion of flowers per inflorescence that set fruit (Note: if we mis-counted some flowers in the inflorescence, then proportion fruit set could be erroneously recorded as a value > 1; this happened rarely).Data for estimation of somatic mutationProportion of flowers setting fruit from autonomously-pollinated inflorescences (Wa), and geitonogamously-pollinated inflorescences (Wg). Also included is the autogamy depression measure. These measurements were paired as described in the article. Clone number refers to a specific clone. There were several Wa and Wg measurements for each clone. The measure D is the autogamy depression measurement, calculated for each paired autogamous and geitonogamous inflorescence cross.Function for calculating the likelihoodUsed for maximum likelihood estimation of somatic mutation parameters.