Data from: Identification of sex-specific molecular markers using restriction site associated DNA sequencing (RAD-seq)
| Main Authors: | Gamble, Tony, Zarkower, David |
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| Format: | info dataset Journal |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4940908 |
Daftar Isi:
- A major barrier to evolutionary studies of sex determination and sex chromosomes has been a lack of information on the types of sex-determining mechanisms that occur among different species. This is particularly problematic in groups where most species lack visually heteromorphic sex chromosomes, such as fish, amphibians and reptiles, because cytogenetic analyses will fail to identify the sex chromosomes in these species. We describe the use of restriction site associated DNA (RAD) sequencing, or RAD-seq, to identify sex-specific molecular markers and subsequently determine whether a species has male or female heterogamety. To test the accuracy of this technique we examined the lizard Anolis carolinensis. We performed RAD-seq on seven male and ten female A. carolinensis and found one male-specific molecular marker. Anolis carolinensis has previously been shown to possess male heterogamety and the recently published A. carolinensis genome facilitated characterization of the sex-specific RAD-seq marker. We validated the male specificity of the new marker using PCR on additional individuals and also found that it is conserved in some other Anolis species. We discuss the utility of using RAD-seq to identify sex determining mechanisms in other species with cryptic or homomorphic sex chromosomes and the implications for the evolution of male heterogamety in Anolis.
- Gamble_Zarkower_Supp_File1Supplementary File 1. RADtools output – full datasetGamble_Zarkower_Supp_File2Supplementary File 2. Fasta file with male-specific RAD lociGamble_Zarkower_Supp_File3Supplementary File 3. RADtools output – sub-sampled datasets