CD163 peripheral inflammatory response in stroke
| Main Authors: | Greco Rosaria, Demartini Chiara, Morotti Andrea, Amantea Diana, Tassorelli Cristina |
|---|---|
| Format: | info dataset Journal |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4916312 |
Daftar Isi:
- This dataset comprises the findings obtained in the study aimed at delineating the innate immune response in ischemic stroke patients. The attention was focused on circulating monocyte subtypes, in relationship to their relevance both in the pathogenesis and as putative therapeutic targets. In particular, we achieved in healthy control subjects and ischemic patients at 24 and 48 hours after stroke the following evaluations: 1)CD163 and CD80 Expression in Peripheral Blood Monocytes (CD14++and CD16+) expressed as percentage of total acquired events by cytofluorimetric analysis. 2)Cytokines (TNF-alpha, IL-1beta, IL-4 and IL-10) Gene Expression in total Monocytes were assayed by rt-PCR. All samples were assayed in triplicate and gene expression levels were calculated according to 2−∆∆Ct = 2− (∆Ct gene − ∆Ct housekeeping gene) formula by using Ct (cycle threshold) values. 3)Correlation between the Percentage of CD163+/CD16+ Events and Stroke Severity at admission.
- Funding: Ricerca Corrente 2017-2019, Ministry of Health (Italy)