In silico protein engineering: Methods and Tools
| Main Authors: | Sneha Thomas, John J. Georrge |
|---|---|
| Format: | Book publication-section Journal |
| Bahasa: | eng |
| Terbitan: |
Christ Publications
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4729855 |
Daftar Isi:
- Protein engineering, the process by which novel proteins with desired properties are developed, has grown by leaps and bounds. An essential objective of protein engineering is the design of peptides that can bind selectively to target proteins. Protein engineering strategies aimed at constructing enzymes with novel or improved activities, specificity, and stabilities greatly benefit from in silico methods such as (i) bioinformatics, (ii) molecular modelling, and (iii) de novo design. Computational approaches offer significant potential for engineering protein structure and function and can be combined with experimental testing to gain new insights into the fundamental properties of proteins. It is based on altering the affinity of a protein by replacing some amino-acid residues to identify the essential residues and their specific involvement in the binding activity. This chapter focuses on the detailed information of the methods and tools of protein engineering and will be beneficial for the researchers pursued in this field