Stimulation of Primula veris seeds' germination under in vivo and in vitro conditions
| Main Authors: | Stanilova Marina, Petrova Maria, Grigorova Irena, Traykova Boryanka, Elina Yankova-Tsvetkova |
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| Format: | Proceeding poster Journal |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4650322 |
Daftar Isi:
- Primula veris L. (Cowslip) is a medicinal plant species with a long history of use, native to Europe and Western Asia. In Bulgaria it is protected by the Biodiversity Act and under special regime of use based on the Medicinal Plants Act. The aim of the present study was to stimulate the extremely low seed germination of P. veris by different approaches. Seeds were gathered from one native population in the Golo Bardo Mountain in Bulgaria, and used for in vivo and in vitro experiments in parallel. The effect of different factors was tested: treatment with plant growth regulators (gibberellic acid, kinetin), light quality (white, infrared, red, and blue) or dark, and stratification at low temperature. Combination of factors resulted in 36 variants in vivo and 8 variants in vitro, about 40 seeds per variant. No germination was observed in control variants without seed treatment. Concerning in vivo variants, the highest germination rates were noticed when seeds were cultivated under red or blue light (70% when seeds were soaked into 0.1% GA3 for 5 h before treatment with red light, and 65% when treated with 0.3% GA3 for 5 h and put under blue light); however, red light caused etiolation and death of seedlings, while blue light stimulated development of large cotyledons and leaves. Among in vitro variants, the most successful factors combination was 2-month seeds stratification at 4 oC followed by cultivation on medium containing 0.5 mg/l kinetin and 5 mg/l GA3 – 50% germinated seeds. In all variants significant number of seeds stopped their development at root stage, even the survival rate varied between variants. Seedlings were transferred consecutively to vermiculite and soil mixture, and currently about hundred plants are growing in the phytotron. These results will be used as a base for establishment of a pilot agriculture of the species.