EMS derived wheat mutant BIG8-1 (Triticum aestivum L.) – a new drought tolerant mutant wheat line
| Main Author: | Anna-Maria Botha |
|---|---|
| Format: | Article Journal |
| Bahasa: | ang |
| Terbitan: |
, 2021
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/4623695 |
Daftar Isi:
- Drought response in wheat is considered a highly complex process since it is a multigenic trait, nevertheless, breeding programs are continuously searching for new wheat varieties with drought-tolerant characteristics. In a previous study, we demonstrated the effectiveness of creating a mutant known as RYNO3936 that could survive 14 days without water, however in this study we revealed another mutant known as BIG8-1 that could endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically the mutant plants had broader leaves including a densely packed fibrous root architecture that were not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, photosynthesis, and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and server (day 21) water deficit stress that a decline in those measurables were evident. Furthermore, the mutant plants also display a unique preservation of metabolic activity, which was confirm when assessing accumulation of free amino acids and increase of antioxidative enzymes (Peroxidases and Glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO, during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1, and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and Xylanase inhibitor protein, which is involved in the transport of amino acids and protecting cells, respectively. Collectively this study demonstrates the characterization of a new mutant BIG8-1, with drought-tolerant characteristics suited for breeding programs.
- None