SELFI single molecule experiment on Alexa647/Phalloidin fluorescence (f-actin labeling) on fibroblast cells
| Main Authors: | Pierre Bon, Jeanne Linarès-Loyez |
|---|---|
| Format: | info dataset |
| Bahasa: | eng |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4518602 |
Daftar Isi:
- These are images of Alexa647/Phalloidin fluorescence (f-actin labeling) on fibroblast cells. Observation at 60x NA=1.4. It is a "typical but not perfect" acquisition + calibration package (near optimal network/detector position). You will find a cell reconstruction image (.tif, the color coding for z) of what is obtained with 750GB of processed data. But as 750Go is not sendable, there is only a part of the images (the first 50 000) to get trained on the image analysis process. The associated calibration images are present in the form "Stack=XXXX_Im=YYYYYY_Z=zzzz.tif" with XXXX the number of the zstack (in this case there are 2 successive z stacks at the same place), YYYYY the ith image at a zzzz position (in nm).