Systematic reanalysis of co-fractionation mass spectrometry data: processed chromatograms
| Main Author: | Skinnider, Michael |
|---|---|
| Format: | info dataset Journal |
| Terbitan: |
, 2021
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4446214 |
Daftar Isi:
- This upload contains processed chromatogram matrices for 206 CF-MS experiments. Database search and protein quantitation was performed with MaxQuant (version 1.6.5.0), and potential contaminants, reverse hits, and proteins identified only by modified peptides were filtered out. Multiple chromatograms are provided for each experiment, corresponding to different protein quantitation strategies. These include iBAQ, MaxLFQ, MS1 intensity, and spectral counts, for label-free datasets, and the isotopologue ratio for SILAC or dimethyl labelling datasets. Each directory also contains a metadata file, which contains further information about each row in the corresponding chromatogram matrices output by MaxQuant. In addition to processed chromatograms, which are provided in the "Chromatograms" directory, the upload also contains the raw output files (proteinGroups.txt) from each MaxQuant search. These files are provided within the "Protein groups" directory. Complete MaxQuant outputs for each experiment are available from the PRIDE repository under the accession PXD022048.