Amyloid tracers binding sites in autosomal dominant and sporadic Alzheimer's disease
| Main Authors: | Ni, Ruiqing, Gillberg, Per-Göran, Bogdanovic, Nenad, Viitanen, Matti, Myllykangas, Liisa, Nennesmo, Inger, Langström, Bengt, Nordberg, Agneta |
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| Format: | Article |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/439367 |
Daftar Isi:
- Introduction: Amyloid imaging has been integrated into diagnostic criteria for Alzheimer's disease (AD). How amyloid tracers binding differ for different tracer structures and amyloid-β aggregates in autosomal dominant AD (ADAD) and sporadic AD is unclear. Methods: Binding properties of different amyloid tracers were examined in brain homogenates from six ADAD with APPswe, PS1 M146V, and PS1 EΔ9 mutations, 13 sporadic AD, and 14 control cases. Results: 3H-PIB, 3H-florbetaben, 3H-AZD2184, and BTA-1 shared a high- and a varying low-affinity binding site in the frontal cortex of sporadic AD. AZD2184 detected another binding site (affinity 33 nM) in the frontal cortex of ADAD. The 3H-AZD2184 and 3H-PIB binding were significantly higher in the striatum of ADAD compared to sporadic AD and control. Polyphenol resveratrol showed strongest inhibition on 3H-AZD84 binding followed by 3H-florbetaben and minimal on 3H-PIB. Discussion: This study implies amyloid tracers of different structures detect different sites on amyloid-β fibrils or conformations.