Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency
| Main Authors: | Ahmed K. Elsayed, Ihab Younis, Gowher Ali, Khalid Hussain, Essam M. Abdelalim |
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| Format: | info dataset Journal |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4362011 |
Daftar Isi:
- Project manager(s): Essam M. Abdelalim Induced pluripotent stem cells (iPSCs) were generated from a patient with a heterozygous deletion of the short arm of chromosome 20 at bands p11.22 to p11.21 (~969 kb deletion), which contains only one gene, FOXA2 (FOXA2+/-iPSCs) as well as healthy controls (Ctr1 iPSCs and Ctr2 iPSCs). FOXA2+/-iPSCs were differentiated into different stages of beta cell development to understand the role of FOXA2 during pancreatic beta cell development as described in the article entitled "Aberrant development of pancreatic beta cells derived from human iPSCs with FOXA2 deficiency" by Elsayed et al. The dataset represents RNA-seq data generated from pancreatic progenitors (PP2) and endocrine progenitors (EPs) derived from Ctr1 iPSCs, Ctr2 iPSCs, and three clones of FOXA2+/-iPSCs. The file name is: Sample name _overall sample number_read direction_001 where: - PP2-Ctr 1: pancreatic progenitors (PP2) derived from Ctr1 iPSCs (healthy control 1) - PP2-Ctr 2: pancreatic progenitors (PP2) derived from Ctr2 iPSCs (healthy control 2) - PP2-FOX1, PP2-FOX2, and PP2-FOX3: pancreatic progenitors (PP2) derived from three different clones of patient-derived FOXA2+/-iPSCs. - EP-Ctr1: endocrine progenitors (Eps) derived from Ctr1 iPSCs (healthy control 1) - EP-Ctr2: endocrine progenitors (Eps) derived from Ctr2 iPSCs (healthy control 2) - EP-FOX R1, EP-FOX R2, and EP-FOX R3: endocrine progenitors (EPs) derived from three different clones of patient-derived FOXA2+/-iPSCs. - The RNA-Seq data were generated from two Ctr-iPSC lines and three FOXA2+/-iPSC lines. - Read direction: R1 (Forward), R2 (Reverse).