The role of lipid peroxidation and antioxidant protection in skin in the development of experimental contact dermatitis
| Main Author: | Regeda-Furdychko, M. M. |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2020
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| Online Access: |
https://zenodo.org/record/4317706 |
Daftar Isi:
- Regeda-Furdychko M. M. The role of lipid peroxidation and antioxidant protection in skin in the development of experimental contact dermatitis. Journal of Education, Health and Sport. 2020;10(12):56-64. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.12.006 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.12.006 https://zenodo.org/record/4317706 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2020; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 09.11.2020. Revised: 25.11.2020. Accepted: 11.12.2020. UDK: 616.5-022.913-092:612.79.014.11].019 THE ROLE OF LIPID PEROXIDATION AND ANTIOXIDANT PROTECTION IN SKIN IN THE DEVELOPMENT OF EXPERIMENTAL CONTACT DERMATITIS M. M. Regeda-Furdychko Lviv Medical Institute Abstract The purpose of work to study the conditions of prooxidant and antioxidant system in the skin of guinea pigs in different periods of contact dermatitis formation. Materials and methods. Researches were conducted on guinea pigs, divided into five groups: I - control, II – 4th day of expirement, III – 8th day of contact dermatitis development, IV – 10th day and V - 18 th day of model process. Early period included groups of animals on the 4th and 8th days of experiment. The late one – guinea pigs on the 10th and 18th days of contact dermatitis. Experimental contact dermatitis was simulated by method of Volkovoj V. A. (2010). Condition of free radical lipid oxidization in the skin was determined on maintenance content of malonic dialdehyde by method of Corobeynikov E. G. (1989) and diene conjugates by method of Gavrylov V. G., Myshkorudna M. I. (1989). The degree of activity of antioxidant defence was estimated on maintenance enzymes - superoxidedismutase by method of R. Fried (1975), catalase by the method of B. Holmes, C. Masters (1970), glutationperoxidase method of Arkhipova O. G. (1988) and glutationreductase method of Moina V. M. (1986). Results and discussion. Gradual increasing of lipid peroxydations products - malonic dialdehyde, diene conjugate had been determined on the 4th, 8th, 10th and 18th days of experimental contact dermatitis development in this research. Primary increasing of the indices of superoxidedismutase, glutationreductase, glutationperoxidase and catalase has been investigated in the skin on the 4th day with the following decreasing of these enzymes from the 8th day of experiment. It testified about imbalance in antioxidant and prooxydant systems, especially on the 10th and 18th days of the study, which exacerbates the inflammatory process in the skin and also triggers a cascade of other mechanisms of damage. Key words: contact dermatitis; malonic dialdehyde; diene conjugate; superoxidedismutase; catalase; glutationperoxidase; glutationreductase.