Aldehyde dehydrogenase 1 (ALDH1) expression in serrated colonic polyps
| Main Authors: | Shyshkin, M. A., Khrystenko, T. O. |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2020
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| Online Access: |
https://zenodo.org/record/4302420 |
Daftar Isi:
- Shyshkin M. A., Khrystenko T. O. Aldehyde dehydrogenase 1 (ALDH1) expression in serrated colonic polyps. Journal of Education, Health and Sport. 2020;10(11):219-225. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.11.021 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.11.021 https://zenodo.org/record/4302420 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2020; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 16.10.2020. Revised: 30.10.2020. Accepted: 30.11.2020. ALDEHYDE DEHYDROGENASE 1 (ALDH1) EXPRESSION IN SERRATED COLONIC POLYPS M. A. Shyshkin, T. O. Khrystenko Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine Shyshkin Maksym Andriiovych, MD, PhD, Associate Professor of the Department of Pathological Anatomy and Forensic Medicine, ZSMU; Maiakovskyi avenue, 26, Zaporizhzhia, 69035, Ukraine. ORCID number 0000-0002-8979-8463, shishkin.stomat@gmail.com Khrystenko Tetiana Oleksiivna, MD, PhD, Assistant of the Department of Pathological Anatomy and Forensic Medicine, ZSMU; Maiakovskyi avenue, 26, Zaporizhzhia, 69035, Ukraine. ORCID number 0000-0003-3364-3339, khristenko.ta@gmail.com Abstract Introduction. Immunohistochemical prognostic markers are promising direction for a search. The purpose of the study was analyze ALDH1 immunohistochemical expression in different histological types of serrated polyps of the distal colon. Materials and methods. Histopathological and IHC studies of 30 serrated polyps were conducted. IHC study was carried out using antibodies against ALDH1. Results of IHC reactions were estimated by photo digital morphometry and were expressed in immunostained cells relative area (%). Results. Hyperplastic polyps are characterized by the median of ALDH1+ stromal cells relative area equal to 15,08 (11,12;21,46) % and the median of ALDH1+ epitheliocytes relative area equal to 18,28 (10,14;26,15) %. The basal-middle pattern of ALDH1+ cells distribution was revealed. Traditional serrated adenomas are characterized by the median of ALDH1+ stromal cells relative area equal to 25,63 (18,26;30,42) % and the median of ALDH1+ epitheliocytes relative area equal to 22,13 (17,22;36,05) %. The upper-middle pattern of ALDH1+ cells distribution was revealed. Sessile serrated adenomas are characterized by the median of ALDH1+ stromal cells relative area equal to 23,15 (17,21;29,14) % and the median of ALDH1+ epitheliocytes relative area equal to 20,56 (15,25;26,94) % in these polyps. The basal-middle pattern of ALDH1+ cells distribution was revealed. Conclusions. Number of ALDH1+ stromal cells is significantly higher in traditional serrated adenomas and sessile serrated adenomas comparing to number of those in hyperplastic polyps. Key words: Intestinal neoplasms; Polyps; Stem Cells; Aldehyde Dehydrogenase 1.