Comparative study of KI-67 and CD44 expression in serrated polyps of the distal colon
| Main Authors: | Shyshkin, M. A., Khrystenko, T. O. |
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| Format: | Article |
| Terbitan: |
, 2020
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| Online Access: |
https://zenodo.org/record/4106980 |
Daftar Isi:
- Shyshkin M. A., Khrystenko T. O. Comparative study of KI-67 and CD44 expression in serrated polyps of the distal colon. Journal of Education, Health and Sport. 2020;10(7):358-365. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.07.038 https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.07.038 https://zenodo.org/record/4106980 The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019. © The Authors 2020; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 19.06.2020. Revised: 09.07.2020. Accepted: 31.07.2020. COMPARATIVE STUDY OF KI-67 AND CD44 EXPRESSION IN SERRATED POLYPS OF THE DISTAL COLON M. A. Shyshkin, T. O. Khrystenko Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine Shyshkin Maksym Andriiovych, MD, PhD, Associate Professor of the Department of Pathological Anatomy and Forensic Medicine, ZSMU; Maiakovskyi avenue, 26, Zaporizhzhia, 69035, Ukraine, shishkin.stomat@gmail.com ORCID number 0000-0002-8979-8463 Khrystenko Tetiana Oleksiivna, MD, PhD, Assistant of the Department of Pathological Anatomy and Forensic Medicine, ZSMU; Maiakovskyi avenue, 26, Zaporizhzhia, 69035, Ukraine, khristenko.ta@gmail.com ORCID number 0000-0003-3364-3339 Abstract Introduction. Serrated polyps are newly distinguished types of colonic polyps. Nowadays lots of questions about serrated neoplastic pathway are still unclear. The purpose of the study was to compare Ki-67 and CD44 immunohistochemical (IHC) expression levels in different histological types of serrated polyps. Materials and methods. Histopathological and IHC studies of 30 serrated polyps were conducted. IHC study was carried out using antibodies against Ki-67 and CD44. Results of IHC reactions with antibodies against Ki-67 were estimated by immunostained nuclei counting and were expressed in proliferation index (PI) while results of IHC reactions with antibodies against CD44 were estimated by photo digital morphometry and were expressed in immunostained cells relative area (%). Furthermore, distribution of Ki-67+ and CD44+ cells in colonic crypts was examined. Results. It was revealed that hyperplastic polyps (HP) were characterized by medium PI and basal-middle pattern of Ki-67+ cells distribution. HP were also characterized by the median of CD44+ cells area equal to 22,36 (13,15;30,41) % and basal-middle pattern of CD44+ cells distribution. Herewith, direct medium strength correlation between Ki-67 and CD44 expression levels in HP was established. Traditional serrated adenomas (TSA) were characterized by medium PI and diffuse pattern of Ki-67+ cells distribution. TSA were also characterized by the median of CD44+ cells area equal to 25,48 (15,19;29,04) % and upper-middle pattern of CD44+ cells distribution. Direct weak strength correlation between Ki-67 and CD44 expression levels in TSA was established. Sessile serrated adenomas (SSA) were characterized by medium PI and basal pattern of Ki-67+ cells distribution. SSA were also characterized by the median of CD44+ cells area equal to 20,54 (11,25;28,15) % and basal-middle pattern of CD44+ cells distribution. Direct medium strength correlation between Ki-67 and CD44 expression levels in SSA was established. Key words: Intestinal neoplasms; Polyps; Cell Proliferation; CD44 Antigen.