Identification and quantitative analysis of furostanol glycosides in caltrop

Main Authors: Burda, Nadiia, Zhuravel, Iryna, Dababneh, Moeen F., Kotov, Andrii, Kotova, Elina, Popyk, Andrii
Format: Article
Terbitan: Bulgarian Pharmaceutical Scientific Society , 2020
Subjects:
Online Access: https://zenodo.org/record/4076011
Daftar Isi:
  • This study describes the identification and quantitative determination of furostanol glycosides in caltrop herb harvested in the fructification period. Furostanol glycosides were identified by thin-layer chromatography (TLC) and quantified by UV-vis spectrophotometry. The furostanol glycosides were visualized on the TLC plate as pink spots after treatment with dimethylamine benzaldehyde solution. UV-vis spectrophotometry quantified these substances to the amount of at least 0.4 %. The data on the identification and quantification of furostanol glycosides obtained in the course of this research was implemented in the development of the Ukrainian State Pharmacopoeia for caltrop herb.