Detection of Resistant-Associated Mutations and Genetic Profile of Resistance to Rifampicin and Isoniazid among Multi-Drug Resistant Mycobacterium tuberculosis Isolates from Plateau State
| Main Authors: | VB Wuyep, A Ani, V Davou, E J Fyaktuand, E. Obishakin, EI Ikeh |
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| Format: | Article |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4007928 |
Daftar Isi:
- ABSTRACT INTRODUCTION: This study was carried out to detect the most common resistant-associated mutations and genetic profile of resistance to rifampicin and isoniazid among MDR. M. tuberculosis isolates. METHODS: A total of 20 MDR M. tuberculosis isolates obtained from presumptive MDRTB cases among new and previously treated case TB in Plateau State by conventional Mycobacteriological techniques. Mycobacterium Tuberculosis Drug Resistant plus line probe assay (MTBDR plus) was used to analyzed the isolates. RESULTS: Findings from this analysis indicated that the most frequent mutation at the rpoB gene occurred at codon (H526Y), that of Kat G mutation occurred at codon (S315T1) while that of inh A gene at codon (C15T). Heterogenic genetic profile of resistance (GPR) rate of 31.9% in the rpoB gene of RIF resistant isolates and homogenic (GPR) of 70% in katG gene of INH resistant isolates were reported in this study. Also, One mutation that occurred as a result of loss of WT8 (S531t) band which is an uncommon mutation was discovered. The result suggests a high degree of genetic variability and frequency of resistant conferring mutation involving codon H526Y of the rpoB gene and codon S531T in Kat G gene. CONCLUSIONS: This study found a high level of heterogeneity and homogeneity in the genetic profile of resistance in rifampicin (RIF) and isoniazid (INH) respectively, which probably may have been responsible for high –level of resistance to RIF than INH by the Mycobacterium tuberculosis Isolates in the study population.