Sex identification of Iraqi local cattle using PCR-RFLP technique
| Main Author: | Zainab S. Al-Allak |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2017
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3937906 |
Daftar Isi:
- This study was conducted in the physiology lab. / department of animal production / college of Agriculture / Misan University and Genetic Engineering lab. / department of biology / college of Science / Misan University. The objective of the study was to identify the sex of bovine embryos using amniotic fluid samples by application of PCR-RFLP assay. Amniotic fluid samples were collected from pregnant uteri of cows brought from the slaughter house (25 samples). DNA was extracted and PCR-RFLP was done using SRY primers, the products of amplification furtherly restricted by (HINDIII) enzyme. The results showed three bands on Agarose gel electrophoresis diagnosed as male samples, while the presence of one band indicated female samples. Amelogenin X was used as internal control which appeared in both sexes but not restricted by the enzyme. These results furtherly confirmed by inspection of embryos anatomy, the results were accurate in 100%. This method will help producers to build a scientific strategy in management with better prediction of sex ratio in the coming season, also prevent slaughter cows pregnant with wanted embryos or vice versa.