A STUDY OF MORPHOLOGY AND BIOCHEMIST OF BACTERIA FOUND IN SHALLOT (Allium ascalonicum L.) RIZOSPHERE WITH PB CONTAMINATION

Main Author: Oetami Dwi Hajoeningtijas*1, Hamami Alfasani Dewanto2, Aman Suyadi3, Agus Mulyadi Purnawanto4
Format: Article
Terbitan: , 2020
Subjects:
Pb
Online Access: https://zenodo.org/record/3908702
Daftar Isi:
  • This research was to find out the effect of heavy metal accumulation in shallot field in Wanasari Subdistrict to the bacteria in shallot rhizosphere. This was a survey with Purposive Random Sampling, employing morphological and biochemical observations to collect the data. The qualitative descriptive method was applied to describe the data of testing results. Based on the results of morphology test, there are 27 various characterizations of bacteria on soil sample taken from the shallot (Allium ascalonicum L.) plantation in Kupu, Wanasari and Sisalem villages. Based on macroscopic, it has round, flat and non flat edges, convex elevation and their colors are all white and clear-white. From the microscopic observation, they have various sizes and their shapes are stem (basil) and round (coccus). The results of a biochemical test of bacteria on 27 isolates showed mixed results of positive and negative for all the tests of Methyl Red, Simon citrate, indole, catalase test, carbohydrate (gas), and a negative results for two tests of Vogeus-proskeur and H2S. The comparison of average Pb content in the soil had proved that the less Pb heavy metal contaminants in the soil means the more bacterial isolates