Real time monitoring of glucose in whole blood by smartphone
| Main Authors: | Erenas, Miguel M, Carrillo-Aguilera, Belén, Cantrell, Kevin, González-Chocano, Sara, Perez de Vargas-Sansalvador, Isabel M, Orbe-Payá, Ignacio de, Capitan-Vallvey, Luis F |
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| Format: | info publication-preprint Journal |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3754651 |
Daftar Isi:
- A combined thread-paper microfluidic device (μTPAD) is presented for the 15 determination of glucose in blood. The device is designed to include all the analytical 16 operations needed: red blood cell separation, conditioning, enzymatic recognition, and 17 colorimetric transduction. The signal is captured with a smartphone or tablet working in 18 video mode and processed by custom Android-based software in real-time. The automatic 19 detection of the region of interest on the thread allows for the use of either initial rate or 20 equilibrium signal as analytical parameters. The time needed for analysis is 12 s using 21 initial rate, and 100 s using the equilibrium measurement with a LOD of 48 μM and 12 22 μM, respectively, and a precision around 7%. The μTPAD allows a rapid determination 23 of glucose in real samples using only 3 μL of whole blood.