Time series measurements of leucine incorporation as a measure of bacterial production in the subtropical North Pacific Ocean
| Main Authors: | Church, Matthew J., Viviani, Donn A. |
|---|---|
| Format: | info dataset |
| Bahasa: | eng |
| Terbitan: |
, 2020
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3712397 |
Daftar Isi:
- Samples were collected at or in the vicinity of Station ALOHA on 24 different cruises. On each cruise, rates of 3H-Leu incorporation into protein were measured. Seawater samples for measurements of production were collected from pre-dawn vertical hydrocasts at 6 discrete depths (5, 25, 45, 75, 100, 125 m) using polyvinyl chloride sampling bottles affixed to a rosette sampler. Polyethylene amber bottles (125 ml capacity) were subsampled from the CTD rosette bottles, and duplicate acid-cleaned 12 ml polycarbonate centrifuge tubes were filled from each depth. Each polycarbonate tube was inoculated with 20 nmol L-1 (final concentration) 3,4,5-3H-leucine. An additional 1.5 ml per depth was subsampled into 2 ml microcentrifuge tubes containing 100 μl of 100% (w/v) ice-cold trichloroacetic acid (TCA) to serve as a killed blank. The polycarbonate sample tubes were capped and incubated in situ over the photoperiod to measure rates of 3H-Leu incorporation in both dark (through use of black cloth bags) and light on a free-drifting array. At the end of the photoperiod, triplicate 1.5 ml subsamples were removed from each tube and added to 2 ml microcentrifuge tubes containing 100 μl of 100% ice-cold TCA; these tubes were stored frozen until analysis. Samples were processed following a modified method of the microcentrifuge method.