Acidifier Affects In Vitro Antimicrobial Effect and In Vivo pH and Intestinal Microflora in Laying Duck
| Main Authors: | Haqza Marufi Ramadhan, Eko Widodo, Irfan H. Djunaidi |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2020
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| Online Access: |
https://zenodo.org/record/3653508 |
Daftar Isi:
- The aims of this study were to evaluate in vitro antimicrobial effect of acidifier and in vivo pH and intestinal microflora in Laying Duck. Acidifier used were formic acid and fumaric acid. This research was divided into two stages. In first experiment, the method used was in vitro experimental method and using Completely Randomized Design (CRD) having 4 treatments and 5 replications. The treatments consisted of T0= aquadest+Bacitracin. T1= aquadest + 0.5% formic acid. T2= aquadest + 0.5% fumaric acid and T3= aquadest + 0.5% mixture of formic acid and fumaric acid at ratio of 50:50. Variables observed were diameter of the clear zone indicating inhibition activity Lactic Acid Bacteria (LAB), Escherichia coli, and Salmonella. At second experiment, in vivo experiment was carried out and using Completely Randomized Design (CRD). One hundred and fifty female ducks of 50 weeks old randomly distributed into 5 treatments and 5 replications. The treatments consisted of T0-= basal feed/control. T0+= basal feed with addition of Bacitracin 0.1%, T1= basal feed + 0.1% mixture of formic acid and fumaric acid, T2= basal feed + 0.2% mixture of formic acid and fumaric acid. T3= basal feed + 0.3% mixture of formic and fumaric acid at ratio of 50:50. The variables observed were population of intestinal pathogenic and non-pathogenic microflora. The result of first experiment showed that clear zone of inhibitory diameter significantly decreased (P>0.01) by any acidifier treatments for type of microbes. In the second experiment, the population of intestinal microflora significantly decreased pH and also significantly decreased population of LAB, Escherichia coli, and Salmonella.