Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework‐8 (ZIF‐8)
| Main Authors: | Arpita Poddar, José J. Conesa, Kang Liang, Sudip Dhakal, Philipp Reineck, Gary Bryant, Eva Pereiro, Raffaele Ricco, Heinz Amenitsch, Christian Doonan, Xavier Mulet, Cara M. Doherty, Paolo Falcaro, Ravi Shukla |
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| Format: | info publication-preprint Journal |
| Terbitan: |
, 2019
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| Online Access: |
https://zenodo.org/record/3647989 |
Daftar Isi:
- Recent work in biomolecule-metal–organic framework (MOF) composites have proven to be an effective strategy for the protection of proteins. However, for other biomacromolecules such as nucleic acids, the encapsulation into MOFs and the related characterizations are at is infancy. We herein report encapsulation of complete gene-set in zeolitic imidazolate framework-8 (ZIF-8) MOFs and intracellular expression of the gene delivered by the MOF composites. Using a GFP plasmid (plGFP) as a proof-of-concept genetic macromolecule, we show successful transfection of mammalian cells with plGFP for up to 4 days, demonstrating the feasibility of DNA@MOF biocomposites as intracellular gene delivery vehicles which occurs over relatively prolonged time points where the cargo nucleic acid is released gradually in order to maintain sustained expression.
- This is the pre-peer reviewed version of the following article: Encapsulation, Visualization and Expression of Genes with Biomimetically Mineralized Zeolitic Imidazolate Framework-8 (ZIF-8), which has been published in final form at http://dx.doi.org/10.1002/smll.201902268. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.