Protein Organization and Pleomorphicity
| Main Authors: | Dr. Abdullah Sethar, Dr. Benjamin W. Neuman |
|---|---|
| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3531352 |
Daftar Isi:
- The result of density transects analysis of the ends (00 and 1800 ) vs sides (900 and 2700 ) of elliptical TCRV are shown in figure 6.7. While the density of the inner and outer membrane peaks remained constant, there was less electron density present in the expected positions of GP, Z and NP on the flat “sides” (900 and 2700 ) of elliptical TCRV particles. The shape of each peak was similar, but no broadening was observed. This was interpreted to indicate that less protein was present at the expected position for each of the three viral structural proteins, GP, Z and NP. It was further revealed understand the role of protein organization in virion morphology, images of TCRV was manually grouped according to the apparent organization of the Z and NP components. In order to do this, all elliptical TCRV particles were selected and manually rotated to match one of the nine categories shown in Figure 6.5. The number of images in each section therefore reflects the prevalence of each type of organization in the population of elliptical TCRV. As shown in Figure 6.5, the most common organization found displayed well organized Z and NP around the termini of the longest diameter, i.e., at highly curved “ends” of each particle. In contrast, Z and NP organization often appeared to be disrupted at the termini of the shortest diameter, i.e., on the less curved “sides” of each particle. The presence of GP appeared to correlate with protein organization, with GP being present only on regions where organized Z and NP were visible inside the virion. Several of the particles appeared to lack GP on the flat or inwardly curving “sides” of the particle