Induction of matrix metalloproteinase'1 in in vitro experimental wound model using a novel three-dimensional culture system
| Main Authors: | Abe M, Kan C, Zaw K K, Ishikawa O |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2001
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| Online Access: |
https://zenodo.org/record/3478845 |
Daftar Isi:
- The aim of this study was to determine whether experimentally punched wounds may influence the connective tissue metabolism of human fibroblast in a three dimensional culture supplemented with L-ascorbic acid. This culture was designed for human dermal fibroblasts to organize a three-dimensional dermis-like structure by accumulating self-produced extracellular matrixes. In order to examine the effects of the wound, mRNA expression and production of type I collagen, matrix metalloproteinase-1 (MMP-1) and tissue inhibitors of metalloproteinase-1 (TIMP-1) were sequentially examined up to 72 hrs after the wound was created.