Perl script for batch processing of ACQUIFER Imaging Machine data via Fiji macros

Main Author: Jochen Gehrig
Format: info software Journal
Bahasa: eng
Terbitan: , 2019
Subjects:
Online Access: https://zenodo.org/record/3373807
Daftar Isi:
  • Batch generation of multilayer tiff files and z-projection for 1-N IM04 datasets. The Perl script will parse all IM04 datasets within a user-specified directory and will generate a Fiji macro to process each unique entry (i.e. imaging coordinate ,or “Coordinate--Subposition--Loop—Colour”). The Fiji macro will subsequently generate colour specific subfolders within each experimental folder. For each “Coordinate--Subposition--Loop—Colour” entry, multilayer tiffs and z-projections are generated. CO6 (=bright field) data will be minimum z-projected, all other channels are maximum z-projected. Tested on Windows OS with Strawberry Perl (http://strawberryperl.com/). Please ignore the first version.