Intestinal barrier dysfunction in hyperoxaluria women with recurrent pyelonephritis
| Main Authors: | Natalia Stepanova, Ganna Tolstanova, Tetyana Sergiychuk, Iryna Akulenko |
|---|---|
| Format: | Proceeding |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3345042 |
Daftar Isi:
- Introduction & Objectives: Nephrolithiasis and prenephrolithiasis states often coexist with recurrent pyelonephritis. The intestine has an important role in the dynamics of oxalate exchange. So, in this way, it is very significant in the etiology of calcium oxalate nephrolithiasis. But, there are a limited number of studies that have analyzed the effects of hyperoxaluria on the gut microbiota composition and intestinal barrier dysfunction in the patients with recurrent pyelonephritis. We hypothesized that the frequent use of antibacterial agents by the patients with the recurrent pyelonephritis could lead to the destruction of the normal composition of gut microbiota with formation of hyperoxaluria and violate intestinal barrier function. The purpose of our work was to investigate the affect of indigenous gut microbiota on the intestinal barrier function in hyperoxaluria patients with recurrent pyelonephritis. Materials & Methods: This observational cross-sectional study represents the data of the microbiological stool examination in 30 hyperoxaluria women with recurrent pyelonephritis and 10 conditionally healthy donors. The overage age of the patients was 37 ± 14.8 years. According to the content of Lactobacillus spp. in the patients’ intestine, the women were allocated into two groups: The 1st Group of the patients (n = 21) had a reduction of Lactobacillus spp. < 10 million CFU / g in faeces, the 2nd one (n = 9) didn’t have any Lactobacillus spp. deficiency. Secretory immunoglobulin A (sIg A) and myeloperoxidase (MPO) activity in coprofiltrate were used as the markers of intestinal barrier function. For the statistical analysis, we used the Student's t-test, nonparametric (U-test) Mann-Whitney and Pearson's rank correlation test. All the statistical analyses were performed using MedCalc. Results: The concentration of sIgA in coprofiltrate in the patients of the 1st Group was significantly increased compared with the women of the 2nd Group: 7.67 [4.08-23.7] vs 4.04 [3.1-4.9] mg / g (p = 0.02 ), but, it did not differ from the results of the control Group: 7.67 [4.08-23.7] vs. 5.14 [2.1-4.9] mg / g (p = 0.27). The presence of inflammatory process in the intestine of the patients with Lactobacillus spp. deficiency confirmed a significantly higher level of MPO activity compared with the 2nd Group patients: 1.93 [1.36-2.45] vs 1.43 [0.69-1. 19] U / g faeces (p = 0.01) and in the comparison with the Control Group: 1.93 [1.36-2.45] vs 1.31 [1.18-1.96] U / g faeces (p = 0.03). Conclusions: The results of this study have provided the preliminary evidence that the hyperoxaluria women with recurrent pyelonephritis have an intestinal barrier dysfunction, which manifests itself by the increasing of sIg A concentration and the MPO activity in patients’ coprofiltrate. The further studies are needed to determine the role of hyperoxaluria in the violation of the intestinal barrier function.