The high level of cytokines associated with hyperoxaluria in the patients with recurrent pyelonephritis
| Main Authors: | Mykola Kolesnyk, Natalia Stepanova, Victoria Driianska, Natalia Stashevska |
|---|---|
| Format: | Proceeding Journal |
| Bahasa: | eng |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3345007 |
Daftar Isi:
- Introduction and objectives: Crystal-cell interaction has been reported as one of the most crucial steps in urinary stone formation. Recent experimental studies have demonstrated the leading role of IL-17 and IL-23 in the pathogenesis of inflammatory bowel disease. Moreover, the expression of intestinal epithelial cells cytokine IL-17 family depend on synanthropic bacteria, namely: The reduction of the total microbiota in the adult mice after the administration of antibiotics has resulted in increased expression of IL-23 and -17 in the colon, suggesting that the synanthropic bacteria are active inhibitors of IL-23 and IL-17. Although, it should be noted that there haven’t been any descriptions of the results of clinical studies on the content of pro- and anti-inflammatory cytokines in the blood and urine in the patients with hyperoxaluria in the scientific literature yet. The aim of our study was to compare the cytokines concentration in the blood and urine in the patients with pyelonephritis depending on the presence of hyperoxaluria. Materials and methods: We evaluated the blood concentration of interleukins (IL) -4, -17, -18, -23 and monocyte chemoattractant protein-1 (MCP-1) and the tumour necrosis factor alpha (TNF-a) level in the urine of 40 white adult women with recurrent pyelonephritis caused by E. coli or S. faecalis. The mean age in thepatient population was 21-48 years (31.6±7.7). Recurrent pyelonephritis was defined as 2 upper urinary tract infection episodes within 6 months or 3 or more episodes during the previous 12 months. According to the presence of hyperoxaluria, the women were allocated into two groups: The first group of the patients (n=29) had hyperoxaluria (>44mg in 24 hours) and the second one (n=11) didn’t have any hyperoxaluria. The cytokines concentrations were analysed using ELISA and STAT FAX-303 PLUS (Diaclon, France; DRG, Germany; Ukrrmedservice, Ukraine). All the statistical analyses were performed using MedCalc. Results: The results of our research have indirectly indicated in favor of the theory mentioned above. The blood levels of IL-4, IL-17 and IL-23 in the women with hyperoxaluria were significantly higher compared with the non-hyperoxaluria patients: 62.2 [52.8-74.1] vs 44.5 [35.8-67] pg/ml (P=0.019), and 130.7 [101.3-231.2] vs 103.4 [77.5-133.9](P=0.03), and 123.2±17.1 vs 80.98±29.4 (P=0.03), respectively. The other mediators (IL-18 and MCP-1) were higher in all of the patients with pyelonephritis in comparison with the group of healthy women, but, they did not differ between the groups. The urine concentration of TNF-a was significantly higher in the patients of Group I compared with the patients of Group II (44 [16.2-130.5] vs 21 [14.2-345] pg/ml; P=0.04). In addition, we identified a moderate direct correlation between the blood level of IL-17 in the patients with recurrent pyelonephritis and daily excretion of oxalate: R=0.54, P=0.03. Conclusions: The results of this study have provided the preliminary evidence that women with hyperoxaluria have high levels of cytokines IL-4, IL-17 and IL-23 in the blood and the urinary excretion of TNF-a. The blood and urinary cytokines concentration in the patients with recurrent pyelonephritis and hyperoxaluriahas has not been scrutinized before. The further studies are needed to determine the role of these cytokines in the progression of urolithiasis in hyperoxaluric conditions.