Production of hyperimmune colostrum in the bovine against cariogenic streptococci and assess its impact on growth and biofilm formation of bacteria
| Main Authors: | Fateme Ramezanalizadeh, Azam Aliasghari, Mohammad Rabbani Khorasgani, Maryam Khoroushi, Arezoo Tahmourethpour, Ahmad Reza Jabari |
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| Format: | Article Journal |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/3342912 |
Daftar Isi:
- Background and Aims: Dental caries is the most common infectious diseases. Among the oral bacteria, Streptococcus mutans and Streptococcus sobrinus are considered as the main causes of tooth decay. The aim of this study was the production of hyperimmune bovine colostrum containing specific antibodies against cariogenic bacteria and evaluation its antimicrobial effects on growth and adhesion of Streptococcus mutans and Streptococcus sobrinus in the laboratory. Materials and Methods: In this experimental study, three pregnant bovine immunized with killed antigens of strains of Streptococcus mutans, Streptococcus mutans with Streptococcus Sobrinus and Streptococcus sobrinus through intramuscular injections. After delivery, The colostrum samples were collected, and the changes of antistreptococci antibodies titers in colostrum and serum determined by agglutination. Also,their antimicrobial effects against the growth and adhesion of oral streptococci were surveyed by microtiter plate method. Results were analysed by One-Wey ANOVA in SPSS software. Results: The results showed that in hyperimmunized bovine , the antibodies titers against injected bacteria were found from 1.1000 to 1.3000 in sera samples and from 1.320 to 1.1280 in whey of colostrum samples. Colostrum of hyperimmune cows reduced attachment of Streptococcus mutans and Streptococcus Sobrinus about 69 and 43 percents respectively and also, the low dilutions of it reduced bacterial growth. Conclusion: According to the antibacterial effect immune colostrum on two strains of cariogenic bacteria in vitro, It appears that this material could be useful in the prevention and control of dental caries.