Raw Diffraction Images: Formation of a highly dense tetra rhenium cluster in a protein crystal and its implications in medical imaging
| Main Authors: | Brink, Alice, Helliwell, John R. |
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| Format: | info dataset |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/2874342 |
Daftar Isi:
- Exploration of a “time on shelf” protein structure containing the radiopharmaceutical synthon fac-[Re(CO)3(H2O)3]+ as an in vivo reaction vessel to form tetranuclear rhenium clusters appropriate for theranostic applications.That a protein crystal can serve as a chemical reaction vessel is intrinsically fascinating. That it can produce an electron dense tetranuclear rhenium cluster compound from a rhenium tricarbonyl tribromo starting compound adds to the fascination. The cluster has been synthesised before in vitro when it formed under basic conditions. Therefore its synthesis in a protein crystal grown at pH4.5 is even more unexpected. The X-ray crystal structures presented here are for the protein hen egg white lysozyme incubated with the rhenium tricarbonyl tribromo compound for periods of 1 year and 2 years. These reveal a completed, very well resolved, tetra rhenium cluster after two years and an intermediate state after 1 year where the carbonyl ligands to the rhenium cluster are not yet clearly resolved. A dense tetra-nuclear rhenium cluster, and its technetium form, offers enhanced medical imaging contrast. The raw diffraction images for the one year and two year protein structure, described in the manuscript, is made avaliable on the Zenodo repository.
- Grant Funding Acknowledgement: South African National Research Foundation (UID 99139)