A complete RNA-sequencing pipeline
| Main Authors: | Rue-Albrecht, Kevin , Nalpas, Nicolas C. |
|---|---|
| Format: | info software |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/18091 |
Daftar Isi:
- First public release of the pipeline used for analysis of RNA-sequencing (RNA-seq) data extended from (Nalpas N.C. et al, http://dx.doi.org/10.5281/zenodo.12474) to include an additional experimental group and novel analyses. Script includes analysis of RNA-seq data from FASTQ files to differential expression, but also gene-set enrichment analysis (GSEA), overlap of differentially expressed gene lists using Venn diagrams, correlation of log2-transformed fold-change value, volcano plots, demonstration of the GOexpress package in Bioconductor, example of a Shiny application to browse the expression data, technical validation using RT-qPCR data and meso-scale discovery (MSD) data.