High-throughput Hisx6-tagged protein purification
| Main Author: | sprotocols |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2015
|
| Online Access: |
https://zenodo.org/record/17567 |
Daftar Isi:
- We have developed a high-throughput protein purification protocol that allows us to purify ~4,000 proteins within 10 hours from prepared culture. By combining steps of cell lysis and protein capture on affinity resins in sealed filter plates, we reduced the number of pipetting steps and thus, human errors.