Protocol for the quantification of protein ng quantities by a Coomassie Brilliant Blue G-250-based hydrophobic assay
| Main Author: | sprotocols |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2015
|
| Online Access: |
https://zenodo.org/record/15896 |
Daftar Isi:
- Several methods for protein determination have been developed [1] but the ones most commonly used today are based on the reaction of proteins with Commassie Brilliant Blue G-250 (CBB) [2] and alkaline Cu(II) [3]. The most recent modifications of these methods are the Sedmak assay [4] and the bicinchoninic acid (BCA) assay [5]. Another sensitive method for measuring proteins is the colloidal gold protein assay [6] which has been modified to quantify specific proteins of interest [7,8].