SNCA targeted gDNA: PacBio raw data
| Main Authors: | Tseng, Elizabeth, Rowell, William J, Chiba-Falek, Ornit, Kujawa, Steve, Glenn, Omolara-Chinue |
|---|---|
| Format: | info dataset |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1560688 |
Daftar Isi:
- The landscape of SNCA transcripts across synucleinopathies: New insights from long reads sequencing analysis gDNA capture using IDT xGen® Lockdown® Probes and single-molecule sequencing 2μg of each gDNA sample was sheared to 6kb using the Covaris g-TUBE and ligated with barcoded adapters. An equimolar pool of 12-plex barcoded gDNA library (2μg total) was input into the probe based capture with a custom designed SNCA gene panel. A SMRTBell library was constructed using 626ng of captured and re-amplified gDNA (https://www.pacb.com/wp-content/uploads/Procedure-Checklist-%E2%80%93-Multiplex-Genomic-DNA-Target-Capture-Using-IDT-xGen-Lockdown-Probes.pdf). A total of 3 SMRT Cells (6 hour movie) were sequenced on the PacBio Sequel platform using 2.0 chemistry. Barcodes used for gDNA demultiplexing bc1001: gcagtcgaacatgtagctgactcaggtcacCACATATCAGAGTGCG bc1002: gcagtcgaacatgtagctgactcaggtcacACACACAGACTGTGAG bc1003: gcagtcgaacatgtagctgactcaggtcacACACATCTCGTGAGAG bc1004: gcagtcgaacatgtagctgactcaggtcacCACGCACACACGCGCG bc1005: gcagtcgaacatgtagctgactcaggtcacCACTCGACTCTCGCGT bc1006: gcagtcgaacatgtagctgactcaggtcacCATATATATCAGCTGT bc1007: gcagtcgaacatgtagctgactcaggtcacTCTGTATCTCTATGTG bc1008: gcagtcgaacatgtagctgactcaggtcacACAGTCGAGCGCTGCG bc1009: gcagtcgaacatgtagctgactcaggtcacACACACGCGAGACAGA bc1010: gcagtcgaacatgtagctgactcaggtcacACGCGCTATCTCAGAG bc1011: gcagtcgaacatgtagctgactcaggtcacCTATACGTATATCTAT bc1012: gcagtcgaacatgtagctgactcaggtcacACACTAGATCGCGTGT
- Three PacBio Sequel 1M cells for targeted gDNA data of SNCA gene in multiplexed (PD, DLB, normal) samples.