HIGHLY SENSITIVE AND RAPID DETECTION OF PUTRESCINE BY USING AN PLATINUM ELECTRODE MODIFIED WITH ZINCOXIDE NANOPARTICLES-POLYPYRROLE COMPOSITE
| Main Author: | Rooma Devi 1*, S.K. Tripathi 1, Aman Chauhan2 & Manju Bala2 |
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| Format: | Article |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1544308 |
Daftar Isi:
- A highly selective, sensitive, specific and rapid electrochemical putrescine biosensor was constructed by covalently immobilizing diamine oxidase purified from Pisum sativum leaves on polypyrrole 3-carboxylic acid (PPy-3C) with zinc oxide nanoparticles ( ZnONps) using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) modified platinum electrode.Transmission electron microscopy (TEM), X-ray diffraction (XRD), UV spectroscopy and Dynamic light scattering (DLS) were used for characterization of ZnONps. The modified enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and electrochemical impedance spectroscopy (EIS).The experimental variables that can affect the putrescine amperometric response, such as the applied potential, pH and temperature have been optimized to perform a selective determination of putrescine.The optimized putrescine biosensor showed linear response range of 0.1–750μM and rapid response within 4s at a potential of 0.4V vs Ag/AgCl. The sensitivity was approximately 40μA/mM/cm2 with a detection limit of 0.1μM. Proposed putrescine biosensor was successfully applied to cancer patients urine sample, which correlated well with a standard enzymic colorimetric method (r = 0.98).