QUALITATIVE AND QUANTITATIVE DETERMINATION OF HUMAN BIOMARKER BY LASER PHOTOACOUSTIC SPECTROSCOPY METHOD
| Main Author: | A. Krishnamanjaripawar, MD.Asif, B. Lakshmiprasanna, T. Samhitha, M.Lavanya |
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| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1400658 |
Daftar Isi:
- Biomarkers can be defined as a characteristic that can objectively measure different biological sample and that can be evaluated as an exposure marker of normal or pathogenic biological processes or of responses to a certain intervention. The biological sample are blood, red blood cells, plasma, serum, urine, nails, saliva, faeces and sample of different tissues. The analysis of breath air has major advantages because it is a non-invasive method, represents minimal risk to personnel collecting the samples and can be often sampled. Breath air samples from the human subjects were collected using aluminized bags from QuinTron and analyzed using the laser photoacoustic spectroscopy (LPAS) technique. LPAS is used to detect traces of ethylene in breath air resulting from lipid peroxidation in lung epithelium following the radiotherapy and also traces of ammonia from patients subjected to hemodialysis for treatment of renal failure. The levels of ethylene and ammonia in exhaled air, from patients with cancer and renal failure, respectively, were measured and compared with breath air contents from healthy humans. Human gas biomarkers were measured at subppb (parts per billion) concentration sensitivities. It has been demonstrated that LPAS technique will play an important role in the future of exhaled breath air analysis. The key attributes of this technique are sensitivity, selectivity, fast and real time response, as well as its simplicity.