ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF ONDANSETRON AND RANITIDINE IN PURE AND ITS TABLET DOSAGE FORM BY RP-HPLC
| Main Author: | CH. Priyanka *, T. Lalitha, B. Madhavi Latha, Dr. D. Satyavati, V. Abhishiktha |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2018
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/1328241 |
Daftar Isi:
- ABSTRACT A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Ondansetron and Ranitidine, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Hypersil C18 (4.6×250mm, 5μ) column using a mixture of Water and Acetonitrile (50:50) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 235nm. The retention time of the Ondansetron and Ranitidine was 2.079, 4.045 ± 0.02 min respectively. The method produce linear responses in the concentration range of 5-25μg/ml of Ondansetron and 93.75-468.75μg/ml of Ranitidine. The method precision for the determination of assay was below 2.0 %RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. KEYWORDS: Ondansetron, Ranitidine, RP-HPLC, validation.