A CROSS-SECTIONAL RESEARCH ON SUSCEPTIBILITY PATTERN OF MYCOBACTERIUM TUBERCULOSIS (MTB) IN SPUTUM SAMPLES
| Main Author: | Dr. Muhammad Naseer ud Din, Dr. Komal Rubab, Dr. Shabana Amir |
|---|---|
| Format: | Article Journal |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1323763 |
Daftar Isi:
- Objective: The research was aimed at knowing the drugs susceptibility pattern in (MTB) i.e. Mycobacterium Tuberculosis in the attendants of multi-drug resistant tuberculosis patients. Methods & Material: The research was performed at Services Hospital, Lahore (August, 16 to March, 17). The research was cross-sectional in that 480 sputum samples from the patients of MDR-TB attendants were under taken for identification of (M. TB). It was performed by Ziehl Neelsen staining, i.e. (BACTEC MGIT-960 culture) line probe assay and also by Lowenstein-Jensen. Results: There were 480 samples, six at 2.1 percent were observed +ve for M. TB by the method of Ziehl Neelsen staining and mean while ten at 2.8 percent were +ve by the use of (LJ & BACTEC MGIT-960 culture). For the aim of taking drugs susceptibility testing, 10 +ve samples were under taken. To find out ethambutol resistant, streptomycin, rifampicin & isoniazid, process of line probes assay test was employed. It was observed that six M. TB isolates were resistant but four were found to be sensitive to both isoniazid & rifampicin. Out of the six resistant M. TB strains, four showed mutations in the “rpo B” gene at three codons as 526, 531 & 516. Conclusion: Most of the attendants of MDR-TB patients had drug-resistant tuberculosis & also the ratio of the drug susceptible T.B was found lesser. Keywords: Tuberculosis, Assay, Sputum, Multi-drug resistant tuberculosis.