BIOCHEMICAL STATUS AND HISTOLOGICAL CHANGES OF CATHARANTHUS ROSEUS ETHANOLIC LEAVES EXTRACT AGAINST MOSQUITO LARVAE AEDES AEGYPTI, ANOPHELES STEPHENSI AND CULEX QUINQUIFASCIATUS (DIPTERA:CULICIDAE)
| Main Author: | V. Shoba |
|---|---|
| Format: | Article Journal |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1314668 |
Daftar Isi:
- The mortality was observed in larvae after 24 h of exposure. The LC50 values were 157.8, 173.1 and 192.3 ppm, respectively. Larvae that were alive after 24 h of extract treatment at LC50 were collected for biochemical and histological examination. The quantitative estimations of glycogen, protein and AchE (brain) was carried out for the Culex quinquifasciatus body tissues of normal and ethanol extract treated larvae of Aedes aegypti, Anopheles stephensi and Cx. quinquifasciatus. The extracts produced significant alterations in the biochemical profiles of Aedes, Anopheles and Culex larvae. Further, the impacting factors of extracts on carbohydrate, protein contents and AchE activity of larvae are species specific. The results indicate altered metabolic activity of the larvae. Histological changes in the mid-gut region of third instar larvae of Cx. quinquifasciatus exposed to LC50 of Catharanthus roseus ethanolic extract presented an irregularly structured brush border after 24 h. The cells began to swell via a slight vacuolization, with disorganized, shortened and confluent microvilli membranes. The structural disorganization of the mid-gut epithelium was evident; cells did not show the characteristic morphology and had destroyed features when compared to control. Based on the results of biochemical status and histological changes of ethanol extracts of C. roseus, it can be concluded that the extract could be a new novel source of various larvicidal active compounds for controlling mosquito vectors.