Antifungal activity of plant extracts and Silver nano particles against Citrus brown spot pathogen (Alternaria citri)
| Main Authors: | Muhammad Farooq, Memoona Siddique, Ateeq –Ur-Rehman, Iltaf Khan, Abdul Saboor, Shoaib Khan, Muhammad Noman Khan, Abdul Qayum, Naila Ilyas, Nabeela Ilyas, Muhammad Bakhtiar |
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| Format: | Article |
| Bahasa: | eng |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1306348 |
Daftar Isi:
- Abstract— Citrus Brown spot caused by Alternaria citri. Different control strategies should be used for management of disease and for increasing potential yield of citrus. Hence in-vitro potential of Plant extract and Silver Nano particles were evaluated for control of Brown spot pathogen in Citrus mangroves of Pakistan. Four different plant extracts garlic, neem, mint and basil leaves extracts were used at concentration of 20%. 40% and 60 %. Their effect on radial mycelial growth was checked with reference to untreated or control petri plate. It was observed that almost all concentrations of plant extracts significantly reduce radial mycelia growth as compared to control. In all treatments of plant extracts it was observed that 60% concentration of neem extracts is effective with radial mycelia growth of 3.96 cm over control petri plate which is 7.73cm, garlic extract@ 60% gave least fungal mycelia growth 5.5cm followed by mint leaves @60% gave 3.16 cm radial growth and Basil leaves extract @60% reduces fungal mycelia growth upto 3.93 cm as compared to control petri plate. Nano particles were used for invitro control of brown spot pathogen .Ten different concentration of nanonparticles were used as 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 ppm. The result of study revealed that by increasing concentration of nano particles inhibit mycelial growth of pathogen more significantly and 100 ppm concentration of Silver nano particles reduce fungal mycelia growth upto 2.63cm as compared to untreated petri plate fungal growth 7.23cm.