Double Stranded RNA Simultaneously Targeting Four White Spot Syndrome Virus (WSSV) genes Provides Protection against WSSV in Litopenaeus Vannamei
| Main Authors: | Anil Kumar, Laramore S, Alexander P, F. C. Thomas Allnutt, Sayre RT |
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| Format: | Article Journal |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1296720 |
Daftar Isi:
- White Spot Syndrome Virus (WSSV) is a major economic threat to global shrimp aquaculture for which there is no available therapeutic. RNA-interference (RNAi)-based strategies could be a promising approach for limiting or curing WSSV epizootics. The present study explored the duration of effective protection provided to shrimp following treatment with long dsRNA molecules targeting WSSV genes. Injection of a mixture of in vitro transcribed dsRNA targeting four WSSV genes (viral capsid protein 28 (vp28), viral capsid protein 19 (vp19), ribonucleotide reductase2 (rr2) and thymidine kinasethymidylate kinase (tk-tmk) provided protection up to 95% for treated shrimp for up to 28 days post-injection. Periodic injection of 2.0 μg of the dsRNA mixture was as effective as injection of a single 15 μg dose in protecting shrimp against WSSV. These data have significant practical implications by providing further knowledge for development of RNAi-based therapeutics against WSSV.