Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14
| Main Authors: | Harding, Rachel, Hutchinson, Ashley, Seitova, Alma, Arrowsmith, Cheryl, Edwards, Aled |
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| Format: | info dataset Journal |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1248166 |
Daftar Isi:
- Project - Huntingtin structure-function open lab notebook. Experiment and rationale - Small scale test-expression of full-length huntingtin Q23 with HAP40 in baculoviral expression system production in sf9 insect cells – 2018/05/14. To validate our insect cell production system for the generation of physiologically relevant huntingtin constructs, HTT and HAP40 will be co-expressed and purified from sf9 culture.
- Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.