Use of antibiotics to optimize micropropagation of Betula pendula var. carelica Merckl
| Main Author: | Kontsevaya I. |
|---|---|
| Format: | Article Journal |
| Bahasa: | rus |
| Terbitan: |
, 2018
|
| Subjects: | |
| Online Access: |
https://zenodo.org/record/1246065 |
Daftar Isi:
- Dependence of the regenerative capacity of single-bud segments of Betula pendula Roth. var. carelica Merckl. shoots on the presence of some antibiotics in a nutrient medium are discussed in the paper. Methods: cell culture in vitro; statistical analysis. Β–lactam antibiotics are optimal at the stage of multiplication of Karelian birch in vitro with use of single–bud shoot segments as explants. Addition of carbenicillin (500 mg/l) and/or cefotaxime (500 mg/l) to the growth medium allows supporting visually clean tissue culture with no significant negative impact on morphometric parameters and viability of micro–plants.
- Работа выполнена при поддержке ГПНИ (No темы М16-33).