DIOSGENIN INDUCES OXIDATIVE STRESS AND APOPTOSIS IN HUMAN HEP-2 LARYNGEAL AND KB ORAL CANCER CELLS
| Main Authors: | P. Suriya, C. Aiyavu, Nethaj, K. Suresh, R. Arunkumar |
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| Format: | Article |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1194882 |
Daftar Isi:
- The present study evaluated the apoptotic effect of Diosgenin on oral cancer cells Hep-2 and KB. Cell growth inhibition and apoptosis effects were demonstrated by nuclear DNA cleavage, condensation, change in membrane potential of mitochondria. Further, apoptosis-related proteins expressions such as mutant p53, Bcl-2, Bax and caspase-3 were determined by Western blotting assays. Results were revealed that, Diosgenin treatment significantly inhibited the cell viability and colony formation, inhibited cell proliferation lead to increased generation of reactive oxygen species through induction of MMP depolarization, morphological changes and DNA damage in dose-and time-dependent manner. Moreover, Diosgenin significantly decreased the Bcl-2 and mutant p53 simultaneously up-regulated Bax, caspase-3 expressions suggest that Diosgenin may serve as a potential candidate in the prevention of cell proliferation and enhances apoptosis.