An Improve Protocol for PCR Using LM1 and LM2 Primers for Listeria monocytogenes Detection in Food Matrices
| Main Authors: | GODÍNEZ-OVIEDO, ANGÉLICA, NAVA, GERARDO M., ARVIZU-MEDRANO, SOFÍA M., HERNÁNDEZ-ITURRIAGA, MONTSERRAT |
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| Format: | Article Journal |
| Terbitan: |
, 2017
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/1185994 |
Daftar Isi:
- Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification of the hlyA gene of Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.